Publications

2018


Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases
Lin L, Liu Y, Xu F, Huang J, Daugaard TF, Petersen TS, Hansen B, Ye L, Zhou Q, Fang F, Yang L, Li S, Fløe L, Jensen KT, Shrock E, Chen F, Yang H, Wang J, Liu X, Xu X, Bolund L, Nielsen AL, Luo Y Gigascience. 2018 Mar 1;7(3):1-19
[ PubMed | Paper ]

Simple method for assembly of CRISPR synergistic activation mediator gRNA expression array
Vad-Nielsen J, Nielsen AL, Luo Y Journal of Biotechnology 2018, 274:54-57
[ Paper ]

Central and Peripheral Nervous System Progenitors Derived from Human Pluripotent Stem Cells Reveal a Unique Temporal and Cell-Type Specific Expression of PMCAs
Chen M, Laursen SH, Habekost M, Knudsen CH, Buchholdt SH, Huang J, Xu F, Liu X, Bolund L, Luo Y, Nissen P, Febbraro F, Denham M Front Cell Dev Biol. 2018 Feb 6;6:5. eCollection 2018
[ PubMed | Paper ]

Induced pluripotent stem cells revolutionise research of neurodegenerative diseases
Schmidt SI, Knudsen MJ, Barnkob HB, Meyer M Ugeskr Laeger. 2016 Jul 18;178(29)
[ Paper ]

Books, Book Chapters


Human Induced Pluripotent Stem Cells and their Derivatives for Disease Modeling and Therapeutic Applications in Alzheimer’s Disease
Pires C, Hall , Freude KK Human Induced Pluripotent Stem Cells and their Derivatives for Disease Modeling and Therapeutic Applications in Alzheimer’s Disease In Alzheimer's Disease. , SMgroup 2016
[ Chapter ]

2017


Neurons derived from sporadic Alzheimer’s disease iPSCs reveal elevated TAU hyperphosphorylation, increased amyloid levels, and GSK3B activation
Ochalek A, Mihalik B, Avci HX, Chandrasekaran A, Téglási A, Bock I, Giudice ML, Táncos Z, Molnár K, László L, Nielsen JE, Holst B, Freude K, Hyttel P, Kobolák J, Dinnyés A Alzheimers Res Ther. 2017 Dec 1;9(1):90
[ PubMed | Paper ]

Generation of high-purity human ventral midbrain dopaminergic progenitors for in vitro maturation and intracerebral transplantation
Nolbrant S, Heuer A, Parmar M, Kirkeby A Nature Protocols 2017, 12(9):1962-1979
[ Paper ]

Reporting on methods to generate and purify rodent and human oligodendrocytes from different sources
Djelloul M, Azevedo C, Pomeshchik Y, Hammarberg A, Roybon L Stem Cell Research 2017, 20:58-66
[ Paper ]

Chromatin accessibility and guide sequence secondary structure affect CRISPR-Cas9 gene editing efficiency
Jensen KT, Fløe L, Petersen TS, Huang J, Xu F, Bolund L, Luo Y, Lin L FEBS Letters 2017, 591(13):1892-1901
[ Paper ]

Communicating Identifiability Risks to Biobank Donors
KASPERBAUER T, GJERRIS M, WALDEMAR G, SANDØE P Cambridge Quarterly of Healthcare Ethics 2017, 27(01):123-136
[ Paper ]

Modeling neurodegenerative diseases with patient-derived induced pluripotent cells: Possibilities and challenges
Poon A, Zhang Y, Chandrasekaran A, Phanthong P, Schmid B, Nielsen TT, Freude KK New Biotechnology 2017, 39:190-198
[ Paper ]

RNA-Guided Activation of Pluripotency Genes in Human Fibroblasts
Xiong K, Zhou Y, Blichfeld KA, Hyttel P, Bolund L, Freude KK, Luo Y Cellular Reprogramming 2017, 19(3):189-198
[ Paper ]

Characterization of energy and neurotransmitter metabolism in cortical glutamatergic neurons derived from human induced pluripotent stem cells: A novel approach to study metabolism in human neurons
Aldana BI, Zhang Y, Lihme MF, Bak LK, Nielsen JE, Holst B, Hyttel P, Freude KK, Waagepetersen HS Neurochemistry International 2017, 106:48-61
[ Paper ]

Patient iPSC-Derived Neurons for Disease Modeling of Frontotemporal Dementia with Mutation in CHMP2B
Zhang Y, Schmid B, Nikolaisen NK, Rasmussen MA, Aldana BI, Agger M, Calloe K, Stummann TC, Larsen HM, Nielsen TT, Huang J, Xu F, Liu X, Bolund L, Meyer M, Bak LK, Waagepetersen HS, Luo Y, Nielsen JE, Holst B, Clausen C, Hyttel P, Freude KK Stem Cell Reports. 2017 Mar 14;8(3):648-658. Epub 2017 Feb 16
[ PubMed | Paper ]

Mitochondrial Spare Respiratory Capacity Is Negatively Correlated with Nuclear Reprogramming Efficiency
Zhou Y, Al-Saaidi RA, Fernandez-Guerra P, Freude KK, Olsen RKJ, Jensen UB, Gregersen N, Hyttel P, Bolund L, Aagaard L, Bross P, Luo Y Stem Cells and Development 2017, 26(3):166-176
[ Paper ]

2016


Creation of a library of induced pluripotent stem cells from Parkinsonian patients
Holmqvist S, Lehtonen Š, Chumarina M, Puttonen KA, Azevedo C, Lebedeva O, Ruponen M, Oksanen M, Djelloul M, Collin A, Goldwurm S, Meyer M, Lagarkova M, Kiselev S, Koistinaho J, Roybon L NPJ Parkinsons Dis. 2016 Jun 2;2:16009. eCollection 2016
[ PubMed | Paper ]

Generation of an isogenic, gene-corrected iPSC line from a symptomatic 57-year-old female patient with frontotemporal dementia caused by a P301L mutation in the microtubule associated protein tau (MAPT) gene
Nimsanor N, Kitiyanant N, Poulsen U, Rasmussen MA, Clausen C, Mau-Holzmann UA, Nielsen JE, Nielsen TT, Hyttel P, Holst B, Schmid B Stem Cell Research 2016, 17(3):556-559
[ Paper ]

Induced pluripotent stem cells (iPSCs) derived from a symptomatic carrier of a S305I mutation in the microtubule-associated protein tau (MAPT)-gene causing frontotemporal dementia
Nimsanor N, Jørring I, Rasmussen MA, Clausen C, Mau-Holzmann UA, Kitiyanant N, Nielsen JE, Nielsen TT, Hyttel P, Holst B, Schmid B Stem Cell Research 2016, 17(3):564-567
[ Paper ]

Generation of an isogenic, gene-corrected iPSC line from a symptomatic 59-year-old female patient with frontotemporal dementia caused by an R406W mutation in the microtubule associated protein tau (MAPT) gene
Nimsanor N, Poulsen U, Rasmussen MA, Clausen C, Mau-Holzmann UA, Nielsen JE, Nielsen TT, Hyttel P, Holst B, Schmid B Stem Cell Research 2016, 17(3):576-579
[ Paper ]

Generation of an isogenic, gene-corrected iPSC line from a pre-symptomatic 28-year-old woman with an R406W mutation in the microtubule associated protein tau (MAPT) gene
Nimsanor N, Poulsen U, Rasmussen MA, Clausen C, Mau-Holzmann UA, Nielsen JE, Nielsen TT, Hyttel P, Holst B, Schmid B Stem Cell Research 2016, 17(3):600-602
[ Paper ]

Generation of induced pluripotent stem cells (iPSCs) stably expressing CRISPR-based synergistic activation mediator (SAM)
Xiong K, Zhou Y, Hyttel P, Bolund L, Freude KK, Luo Y Stem Cell Research 2016, 17(3):665-669
[ Paper ]

Induced pluripotent stem cell - derived neurons for the study of spinocerebellar ataxia type 3
Hansen SK, Stummann TC, Borland H, Hasholt LF, Tümer Z, Nielsen JE, Rasmussen MA, Nielsen TT, Daechsel JC, Fog K, Hyttel P Stem Cell Research 2016, 17(2):306-317
[ Paper ]

Derivation of induced pluripotent stem cells from a familial Alzheimer's disease patient carrying the L282F mutation in presenilin 1
Poon A, Li T, Pires C, Nielsen TT, Nielsen JE, Holst B, Dinnyes A, Hyttel P, Freude KK Stem Cell Research 2016, 17(3):470-473
[ Paper ]

Generation of a gene-corrected isogenic control hiPSC line derived from a familial Alzheimer's disease patient carrying a L150P mutation in presenilin 1
Poon A, Schmid B, Pires C, Nielsen TT, Hjermind LE, Nielsen JE, Holst B, Hyttel P, Freude KK Stem Cell Research 2016, 17(3):466-469
[ Paper ]

Generation of a gene-corrected isogenic control cell line from an Alzheimer's disease patient iPSC line carrying a A79V mutation in PSEN1
Pires C, Schmid B, Petræus C, Poon A, Nimsanor N, Nielsen TT, Waldemar G, Hjermind LE, Nielsen JE, Hyttel P, Freude KK Stem Cell Research 2016, 17(2):285-288
[ Paper ]

Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line SCA3.A11
Hansen SK, Borland H, Hasholt LF, Tümer Z, Nielsen JE, Rasmussen MA, Nielsen TT, Stummann TC, Fog K, Hyttel P Stem Cell Research 2016, 16(3):553-556
[ Paper ]

Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line SCA3.B11
Hansen SK, Borland H, Hasholt LF, Tümer Z, Nielsen JE, Rasmussen MA, Nielsen TT, Stummann TC, Fog K, Hyttel P Stem Cell Research 2016, 16(3):589-592
[ Paper ]

The positional identity of iPSC-derived neural progenitor cells along the anterior-posterior axis is controlled in a dosage-dependent manner by bFGF and EGF
Zhou S, Ochalek A, Szczesna K, Avci HX, Kobolák J, Varga E, Rasmussen M, Holst B, Cirera S, Hyttel P, Freude KK, Dinnyés A Differentiation 2016, 92(4):183-194
[ Paper ]

Generation of a human induced pluripotent stem cell line via CRISPR-Cas9 mediated integration of a site-specific homozygous mutation in CHMP2B
Zhang Y, Schmid B, Nielsen TT, Nielsen JE, Clausen C, Hyttel P, Holst B, Freude KK Stem Cell Research 2016, 17(1):151-153
[ Paper ]

Generation of a human induced pluripotent stem cell line via CRISPR-Cas9 mediated integration of a site-specific heterozygous mutation in CHMP2B
Zhang Y, Schmid B, Nielsen TT, Nielsen JE, Clausen C, Hyttel P, Holst B, Freude KK Stem Cell Research 2016, 17(1):148-150
[ Paper ]

Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a L150P mutation in PSEN-1
Tubsuwan A, Pires C, Rasmussen MA, Schmid B, Nielsen JE, Hjermind LE, Hall V, Nielsen TT, Waldemar G, Hyttel P, Clausen C, Kitiyanant N, Freude KK, Holst B Stem Cell Research 2016, 16(1):110-112
[ Paper ]

Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying an A79V mutation in PSEN1
Li T, Pires C, Nielsen TT, Waldemar G, Hjermind LE, Nielsen JE, Dinnyes A, Hyttel P, Freude KK Stem Cell Research 2016, 16(2):229-232
[ Paper ]

Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a M146I mutation in PSEN1
Li T, Pires C, Nielsen TT, Waldemar G, Hjermind LE, Nielsen JE, Dinnyes A, Holst B, Hyttel P, Freude KK Stem Cell Research 2016, 16(2):334-337
[ Paper ]

Generation of an isogenic, gene-corrected control cell line of the spinocerebellar ataxia type 2 patient-derived iPSC line H266
Marthaler AG, Tubsuwan A, Schmid B, Poulsen UB, Engelbrecht AF, Mau-Holzmann UA, Hyttel P, Nielsen TT, Nielsen JE, Holst B Stem Cell Research 2016, 16(1):202-205
[ Paper ]

Generation of spinocerebellar ataxia type 2 patient-derived iPSC line H196
Marthaler AG, Schmid B, Tubsuwan A, Poulsen UB, Hyttel P, Nielsen TT, Nielsen JE, Holst B Stem Cell Research 2016, 16(1):199-201
[ Paper ]

Generation of an isogenic, gene-corrected control cell line of the spinocerebellar ataxia type 2 patient-derived iPSC line H271
Marthaler AG, Schmid B, Tubsuwan A, Poulsen UB, Engelbrecht AF, Mau-Holzmann UA, Hyttel P, Nielsen JE, Nielsen TT, Holst B Stem Cell Research 2016, 16(1):180-183
[ Paper ]

Generation of spinocerebellar ataxia type 2 patient-derived iPSC line H266
Marthaler AG, Schmid B, Tubsuwan A, Poulsen UB, Hyttel P, Nielsen TT, Nielsen JE, Holst B Stem Cell Research 2016, 16(1):166-169
[ Paper ]

Generation of an isogenic, gene-corrected control cell line of the spinocerebellar ataxia type 2 patient-derived iPSC line H196
Marthaler AG, Schmid B, Tubsuwan A, Poulsen UB, Engelbrecht AF, Mau-Holzmann UA, Hyttel P, Nielsen JE, Nielsen TT, Holst B Stem Cell Research 2016, 16(1):162-165
[ Paper ]

Generation of spinocerebellar ataxia type 2 patient-derived iPSC line H271
Marthaler AG, Tubsuwan A, Schmid B, Poulsen UB, Hyttel P, Nielsen JE, Nielsen TT, Holst B Stem Cell Research 2016, 16(1):159-161
[ Paper ]

Induced pluripotent stem cells (iPSCs) derived from a pre-symptomatic carrier of a R406W mutation in microtubule-associated protein tau (MAPT) causing frontotemporal dementia
Rasmussen MA, Hjermind LE, Hasholt LF, Waldemar G, Nielsen JE, Clausen C, Hyttel P, Holst B Stem Cell Research 2016, 16(1):105-109
[ Paper ]

Neurosphere Based Differentiation of Human iPSC Improves Astrocyte Differentiation
Zhou S, Szczesna K, Ochalek A, Kobolák J, Varga E, Nemes C, Chandrasekaran A, Rasmussen M, Cirera S, Hyttel P, Dinnyés A, Freude KK, Avci HX Stem Cells Int. 2016;2016:4937689. Epub 2015 Dec 21
[ PubMed | Paper ]

Genetically modified pig models for neurodegenerative disorders
Holm IE, Alstrup AKO, Luo Y J Pathol. 2016 Jan;238(2):267-87. Epub 2015 Nov 28
[ PubMed | Paper ]

2015


Impaired APP activity and altered Tau splicing in embryonic stem cell-derived astrocytes obtained from an APPsw transgenic minipig
Hall VJ, Lindblad MM, Jakobsen JE, Gunnarsson A, Schmidt M, Rasmussen MA, Volke D, Zuchner T, Hyttel P Dis Model Mech. 2015 Oct 1;8(10):1265-78. Epub 2015 Aug 6
[ PubMed | Paper ]

Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models
Djelloul M, Holmqvist S, Boza-Serrano A, Azevedo C, Yeung M, Goldwurm S, Frisén J, Deierborg T, Roybon L Stem Cell Reports. 2015 Aug 11;5(2):174-84. Epub 2015 Jul 30
[ PubMed | Paper ]